FEMS Immunology and Medical MicrobiologyLatest Issue | Online Early | The Publisher | Editorial Board
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Chief Editor: Patrik Bavoil
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21% increase of the 2009 Impact factor: 2.335
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We offer rapid review and publication of top class research in fields relating to immunology, medical microbiology, cell biology (of infectious diseases), and the biochemistry, molecular biology and genetics of pathogens.
All articles are published FREE of charge.
Colour is free - except for non-essential colour in print.
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Acces freely here the latest thematic issue on
NEGLECTED TROPICAL DISEASES |
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Highlight: STxA-deficient OMVs from E. coli O157:H7
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Shiga toxin A subunit mutant of Escherichia coli O157:H7 releases outer membrane vesicles containing the B-pentameric complex Sang-Hyun Kim , Sang-Rae Lee, Keun-Su Kim, Ara Ko, Ekyune Kim, Yong-Hwan Kim & Kyu-Tae Chang
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Shiga toxins (STx) are secreted extracellularly through the outer membrane vesicles (OMVs) of Escherichia coli O157:H7. In an attempt to produce STxA-deficient OMVs from E. coli O157:H7, site-specific deletions of the stx1A and stx2A subunit genes were carried out. The STxA-deficient phenotype of the stx1A/stx2A mutant was confirmed by Vero cell cytotoxicity and VTEC-RPLAŽ assay. Western blot analyses showed that the B (STxB) subunits were present without coupling to STxA in the OMVs of the STxA-deficient mutant. Furthermore, STxB was located in its homo-pentameric complexes, as revealed by immunoprecipitation and immunoblotting with anti-STxB antibodies. These results suggest that STxB alone can be oligomerized into the B pentamer in the periplasm, and subsequently entrapped into the OMVs. Determination of the median lethal dose concentration for the OMV preparations suggests that the STxA-deficient OMVs containing STxB complex could be safely used as vaccine delivery vehicles.
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The editors of FEMS Immunology and Medical Microbiology aim to publish outstanding primary Research Articles and MiniReviews reporting on hypothesis-driven studies relating to infection, infection control and their molecular and cellular correlates. The infection typically involves that of humans or animals by microorganisms of all classes, i.e. viruses, bacteria, fungi or protozoa. The scientific approaches of these studies correspond broadly to the fields of immunology, medical microbiology, cell biology (of infectious diseases), and the biochemistry, molecular biology and genetics of pathogens. These include prominently the overlapping subspecialties of molecular and cellular microbial pathogenesis, host innate and adaptive immune responses to infection, "-omics" of pathogens and/or of the infected host, and modeling of the infection or disease (from biomathematical to in vitro to animal modeling). The Journal will also consider outstanding vaccine-related studies and molecular diagnostic and epidemiology studies that are focused on the infectious agent or the infection process.
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