In 2017, we awarded Research and Training Grants on topics as diverse as Yeast-bacterial interactions and Uncovering the microbiology and kinetics underpinning the anaerobic digestion of lipids. The experiences of some of our grantees are highlighted below. Full details of all FEMS Research and Training Grantees whose applications were approved in 2017, can be found here. Details of grantees from previous years are below.
“My research area is Computational Biology and Bioinformatics, as applied to the study of bacterial genomics. This grant allowed me to expand my area of expertise and provided me with a unique and exciting opportunity to join an extremely productive research group which is at the forefront of investigations on comparative and functional genomics of bifidobacteria. My research stay in the Laboratory of Probiogenomics at the University of Parma, Italy, generated new insights and opportunities for future research, and also allowed me to bolster an existing collaboration between University College Cork/APC Microbiome Ireland and the University of Parma.
For this reason I strongly encourage all young researchers to avail of this invaluable opportunity FEMS offer of promoting collaborative exchange of expertise between international research teams, which can bring new insights and inspiration in future research.”
Dr Francesca Bottacini , University College Cork, Ireland (IT-SIM)
“I am a PhD student at the Slovak Academy of Sciences, focusing on lipid biochemistry and genetics of the yeast Saccharomyces cerevisiae, more precisely working to understand an important phenomenon known as lipotoxicity. Thanks to FEMS Research grant, I had the opportunity to vist Prof. Roger Scheniter’s lab at the University of Fribourg, Switzerland.
During my stay I worked in this excellent laboratory, learned a lot of new techniques and extended my knowledge about the yeast lipid metabolism. This grant made possible a collaboration, which has helped me to move our research forward.
Zsófia Csáky, Slovak Academy of Sciences, Slovakia (CZ&SK-CSSM)
|Research and training grantees|
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.