Sign up here to get involved…

… AND OVERCOME THE HURDLES IN THE LIFE CYCLE OF A MICROBIOLOGIST!

* indicates required



I’d like to get involved and:

I want to receive emails from the FEMS Journals

If you subscribe to our newsletter and/or want to know more about the opportunities board, volunteering or interning at FEMS, we will send you an email to confirm your registration and to guide you through the follow-up steps.

If you sign up for alerts from the FEMS Journals, Oxford University Press will register your details for an Oxford Academic account and send you an email to confirm your registration. By completing this section of the form I consent to signing up to an Oxford Academic Account. I agree to the terms as stated at global.oup.com/privacy.

Featured article

A protocol for multiple genetic modifications in S. cerevisiae using CRISPR/Cas9

Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.

read more
More articles
more articles