EAM
During the course of the past 40 years, we have launched and developed five highly-respected journals, which largely fund our charitable activities. This includes our grants programme, biennial congress, and the European Academy of Microbiology (EAM).
The EAM is a leadership group of around 150 eminent microbiology experts who came together in 2009 to amplify the impact of microbiology and microbiologists in Europe.
Past activities
| Year | Key initiatives EAM and EMF have organized |
| 2011 | Article – the enemy within us: lessons from the 2011 European Escherichia coli O104:H4 outbreak |
| 2011 | Workshop – participation at the FEMS Congress 2011 included a 2 hour EAM/EMF-led Keynote Lecture and Roundtable Discussion on “Emerging Diseases — Pandemics, Drug Resistance and Vaccines” |
| 2012 | Seminar – a seminar addressing Coinfections was hosted by the European Academy of Microbiology (EAM) and the German National Academy of Sciences in Leopoldina. Ten speakers presented. A Special Edition ‘Co-infections’ was produced by the EAM and EMF in FEMS Pathogens and Disease Journal. It included a total of 10 x mini reviews / research articles. |
| 2013 | Workshop – Infection Diagnostics and Infection Biology |
| 2014 | Workshop – a retreat workshop with a number of presentations |
| 2015 | Special event – at FEMS 2015, EAM chaired the special event ‘Microbiome and human health’ |
| 2016 | Workshop – a retreat workshop with a number of presentations |
A protocol for multiple genetic modifications in S. cerevisiae using CRISPR/Cas9
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.
