The Federation of European Microbiological Societies is governed by a Council of Member Society Representatives (Delegates) and a Board of Directors. The FEMS Team runs its operations from Delft, The Netherlands. The FEMS Council meets annually with participants consisting of Delegates from all Member Societies, alongside officers of the Board of Directors. The Council Meetings and the 43rd Annual General Meeting were held in Lisbon, Portugal on 8-9 September 2017.
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.