Events
Cracking the Code of AMR in Water with Digital PCR
Summary
Antimicrobial resistance (AMR) is a global concern. Understanding the spread of resistance genes in the environment is essential for monitoring and intervention. In this study, we focus on the detection of antibiotic resistance genes (ARGs) in hospital wastewater, urban surface water, and effluent using digital PCR (dPCR). For this purpose, we developed three multiplex assays allowing the analysis of a total of twelve ARGs (eight carbapenemase and four ESBL genes). The results reveal clear differences in ARG abundance between the different water streams. These insights contribute to a better understanding of how AMR spreads through water. The study is part of the SPOWAR project, which aims to develop new technologies to reduce AMR microorganisms and persistent substances in (waste)water.
Speakers
Carmen holds a Bachelor’s degree in Biology and Medical Laboratory Research and began her career as a technician in a diagnostic microbiology laboratory, where she first specialized in bacteriology before moving to the molecular department. While working, she pursued a part-time Master’s degree in Molecular Life Sciences. After completing her Master’s, she joined a research institute as a research technician and is now pursuing a PhD focused on safeguarding water resources through molecular epidemiology of infectious diseases and antimicrobial resistance. Her doctoral research investigates antimicrobial resistance in (waste)water, with a particular emphasis on digital PCR as a key analytical tool.