FEMS serves the European microbiological community by presenting a comprehensive list of microbiology events. The calendar focuses on international events throughout Europe and also includes the FEMS Meetings.
The calendar is updated on a regular basis. Please feel free to inform us about relevant meetings we missed and post your event on our website.
|upcoming microbiology EVEnts|
|ALL MICROBIOLOGY EVENTS|
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Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.