Microbiology & Infection

 

FEMS in association with two FEMS Member Societies, the Association for General and Applied Microbiology (VAAM) and the German Society for Hygiene and Microbiology (DGHM) have produced a collection of articles called “Microbiology & Infection” in connection with the 5th Joint Conference VAAM Annual Meeting 2017 • 69th Annual Meeting of the DGHM 5–8 March 2017 • Würzburg (Germany).

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Association for General and Applied Microbiology (VAAM)

More than 3500 microbiologists are members of the Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM, Association for General and Applied Microbiology). The VAAM promotes the exchange of scientific information and translates the benefits of microbiological research for the society and the environment. This is achieved by the annual conference in spring representing all areas of microbiology, as well as through special conferences on specific microbiological topics. The members’ journal BIOspektrum is published seven times a year, offering the full spectrum of biological sciences.

German Society for Hygiene and Microbiology

The German Society for Hygiene and Microbiology is one of the oldest specialized societies in the areas of recognition, treatment and prevention of infectious diseases. The affiliated International Journal of Medical Microbiology publishes research articles and reviews dealing with molecular mechanisms of pathogenicity and evolution of pathogens

Federation of European Microbiological Societies

FEMS,  the Federation of European Microbiological Societies – promotes excellence and diversity in science to help solve societal problems around microbiology. It does this by funding research, publishing journals, and by building a growing, collaborative network

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Featured article

A protocol for multiple genetic modifications in S. cerevisiae using CRISPR/Cas9

Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.

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