Letters Incubator

Fostering new ideas and talent

The Letters Incubator builds on the long history of FEMS Microbiology Letters in nurturing new research talent and fostering new innovative ideas in microbiology. It offers the opportunity for key opinion leaders to develop new publishing ideas while mentoring the future talent of the microbiology community.

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Featured article

A protocol for multiple genetic modifications in S. cerevisiae using CRISPR/Cas9

Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.

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