Fostering new ideas and talent
The Letters Incubator builds on the long history of FEMS Microbiology Letters in nurturing new research talent and fostering new innovative ideas in microbiology. It offers the opportunity for key opinion leaders to develop new publishing ideas while mentoring the future talent of the microbiology community.
- Explore the Thematic Series that are currently underway with the Letters Incubator and find out more about the guest editors.
- If you have new publishing ideas, we would like to hear from you. Please complete the Letters Incubator proposal form to get started. If you have any questions about the Letters Incubator, please contact Erwin Ripmeester for more information.
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.