Accelerating the Discovery of New Antimicrobial Compounds
The increasing occurrence of multidrug resistant (MDR) bacteria is driving an urgent demand for the discovery of new antibiotics. The incidence of MDR bacteria is becoming a serious problem, especially in the hospital setting. High-throughput screening methodologies are important to speed up the discovery of new antibiotics. Automation and miniaturization are important components in this process, since they significantly decrease the amount of drugs and reagents needed.
This webcasts, organized by Springer Nature, with two speakers, Riccardo Russo (PhD., MBA. Rutgers University) and Ian Russell (PhD. Labcyte) and with Dr Jayshan Carpen (Nature Research) as moderator, will present some of the main assays used in the early stages of the antimicrobial compound discovery:
(1) Minimal Inhibitory Concentration (MIC) assay,
(2) synergy assay among 2 or 3 antimicrobial compounds,
(3) determination of the concentration of bacteria in liquid cultures,
(4) cytotoxicity assay and (5) bacterial growth/killing curves.
During the webcast they will discuss why and how they developed, and optimized protocols for the miniaturization and automation of these assays using the Echo Liquid Handler. The protocols developed have increased our capacity for screening new antimicrobial compounds by 400%, decreased the time for the preparation of several assays from several hours to minutes and allowed to routinely run assays that were not possible with a large number of compounds due to time and labour constrains.
Date: 26 April 2018
Time: 8am PDT / 11am EDT / 4pm BST / 5pm CEST