Postdoc in genetic engineering of autotrophic bacteria: Sweden
This project will create new metabolic pathways for production of biochemicals, and to test these pathways in a non-photosynthetic, CO2-fixing bacteria. In one part of the project, mutagenesis “libraries” of the host strain will be created and screened in high-throughput, using state-of-the-art techniques. Systems biology analysis of selected strains, such as proteomics and metabolomics, will be used to identify flux bottlenecks in conversion of CO2 to biochemical. This project has a large experimental component. Metabolic modeling will be done with computational biologists in the group. The project is part of an international collaboration between groups in metabolic engineering, metabolic modeling, and enzymologists at KTH, EPFL, and Greifswald. The applicant will therefore work closely with experts in these areas.
Work is in the research group of Paul Hudson in the school of Chemistry, Biotechnology, and Health at KTH. Our group is located at Science for Life Laboratory. The group works on systems biology and metabolic engineering of autotrophic bacteria. See also https://www.kth.se/profile/huds
- Ph.D. in Biotechnology, Chemical Engineering, Biochemistry, or similar
- Good/excellent knowledge in English and a good/excellent ability to express themselves in both speech and writing
- Experience in molecular cloning of metabolic pathways, for example the Gibson Assembly method
- Experience in cultivation of microbes
- Knowledge of microbial physiology
Other qualifications (meriting)
- Experience with CRISPR/Cas9 mutagenesis
- Experience with HPLC or similar analytical technique.
- Experience with analyzing NGS data using R or Python
- Great emphasis will be placed on personal competence and suitability
- Independence in planning and executing experiments
- Ability to communicate research
- Ability to work in multidisciplinary team