Joint Special Issues
The Publications Network shares publication expertise and develops cross-promotional opportunities for Member Societies who publish journals, books and any other publications.
The first publication by this network is the Virtual Special Issue Europe’s Microbiology Network. This virtual special issue showcases a selection of the best research recently published in some of the journals produced by Societies in the FEMS microbiology network.
The second publication is a collection of articles called Microbiology & Infection, is by FEMS in association with two FEMS Member Societies, the Association for General and Applied Microbiology (VAAM) and the German Society for Hygiene and Microbiology (DGHM).
The third publication called Microbiology in Action is a collaboration with seven Member Societies. This collection showcases the best of microbiology from our Member Societies.
The fourth publication called Microbiology in Action II is a sequel of Microbiology in action and a collaboration with eight of our Member Societies.
To get involved with the Publications Network contact Dr Erwin Ripmeester.
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.