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We work to promote excellence in science and to maximize the impact of microbiology and microbiologists. As part of this, we work with a growing body of microbiology experts, some of whom are part of scientific academies and organizations worldwide.
Click on the academies and organizations below to meet the microbiologists we work with in tackling some of the world’s most pressing microbiology-related challenges.
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|Academy of Europe||Estonian Academy of Sciences||Royal Swedish Academy of Sciences||EMBO|
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.