Partnering with FEMS

FEMS is a unique, active and diverse network of around 30,000 professionals who are committed to advancing microbiology for the benefit of society in the areas of health, energy, food, materials and the environment. Set up in the UK as a small charity in 1974, today we are a growing coalition of 54 Member Societies from 38 countries across Europe. Many of those in our network are early career researchers. Others are experienced scientists, industry professionals, students and campaigners.

See our Partnership Opportunities handout for more information.

Featured article

A protocol for multiple genetic modifications in S. cerevisiae using CRISPR/Cas9

Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.

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