Do you know anyone – either an individual or a group – that has provided outstanding service to microbiology in Europe? Have they done something that deserves recognition? Then why not nominate them for the FEMS-Lwoff Award? Winners receive:
- a prize-lecture at the opening ceremony of FEMS 2019 – with up to five free registrations to the FEMS Congress
- the opportunity to present research to the wider microbiology community via the FEMS Journals and FEMS communication channels
- a commemorative silver medal
- an honorarium of €1.000
Everyone in the field of microbiology (societies, groups, or individuals) may nominate a Lwoff Award candidate to be presented at the 2021 Congress before 10 of March 2020.
About the Lwoff Award
Launched in 2000, this award aims award those that create high quality knowledge that helps solving today’s societal problems around microbiology. It was named in honour of the 1st FEMS President (1974-1976), Professor André M. Lwoff.
Making a nomination
Additional information about the selection procedure can be found in the regulations and nomination details.
You can send your nomination, including the requested information to email@example.com using the header: Lwoff Award nomination.
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.