The FEMS Council meets annually. It consists of the Delegates of all Member Societies and the officers of the Board of Directors. Members of the Council who are unable to attend may appoint a proxy/deputy. This should be done by using the relevant form (see below). All members of the Council wishing to attend the meeting should complete a registration form (see below) and make their own travel and accommodation arrangements. FEMS Council Participation Grants are available to support the attendance of Delegates of Full Member Societies who are unable to provide financial support themselves. Member Societies may bid to host the Council meeting in their own country. The Board of Directors will elect one venue from amongst those bids.
Documents from these meetings are available at the FEMS Delegates pages (restricted access) or can be retained by FEMS Delegates from our office.
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.