Out of the FEMS Research and Training Grantees, the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) annually selects one grantee for an additional award of € 1000, the FEMS-ESCMID Awardee. Reciprocally, FEMS will provide such additional award to one grantee, the ESCMID-FEMS Awardee. These joint fellowships will receive appropriate publicity through both organizations.
Two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could contain up to two gRNAs was used to simultaneously introduce up to six genetic modifications (e.g. six gene deletions) in a single transformation step by transforming up to three gRNA expression plasmids simultaneously. The method is not only suitable for gene deletion but is also applicable for in vivo site-directed mutagenesis and integration of multiple DNA fragments in a single locus.